Cooked Meat Medium with Glucose, Hemin, and Vitamin K

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cooked Meat Medium with
Glucose, Hemin, and Vitamin K

培养基配方
Composition per liter:
Heart tissue granules.................................................................98.0g
Peptic digest of animal tissue ....................................................20.0g
NaCl..........................................................................................5.0g
Glucose .....................................................................................5.0g
Yeast extract..............................................................................5.0g
Hemin.....................................................................................5.0mg
Vitamin K.................................................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add approximately 1.0g of heart tissue
granules to test tubes. Add remaining components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Distribute into
tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–
121°C. Slowly cool tubes to prevent expulsion of meat particles.
Use: For the cultivation of anaerobes, especially pathogenic
Clostridia.