Converse Liquid Medium, Levine Modification

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Converse Liquid Medium, Levine Modification

培养基配方
Composition per liter:
Ionagar No.2 or Noble agar...................................................10.0g
Glucose.......................................................................4.0g
Ammonium acetate.............................................................1.23g
K2HPO4 ......................................................................0.52g
Tamol.........................................................................0.5g
MgSO4 ·7H2O..................................................................0.4g
KH2PO4 .......................................................................0.4g
NaCl........................................................................0.014g
Na2CO3 .....................................................................0.012g
CaCl2 ·2H2O ...............................................................0.002g
ZnSO4 ·7H2O................................................................0.002g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes in 15.0mL volumes.
Use: For the cultivation and induction of spherules of Coccidioides
immitis.