Chromogenic Salmonella Esterase Agar (CSE Agar)

[所属分类:培养基配方] [发布时间:2020-4-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromogenic Salmonella Esterase Agar
(CSE Agar)

培养基配方
Composition per liter:
Agar.......................................................................12.0g
Lactose....................................................................14.65
Peptone ....................................................................4.0g
Tryptone....................................................................4.0g
Tween™ 20...................................................................3.0g
Lab Lemco...................................................................3.0g
Na3 -citrate dihydrate......................................................0.5g
L -cysteine...............................................................0.128g
Tris.......................................................................0.06g
SLA-octonoate solution....................................................50.0mL
Novobiocin solution.......................................................10.0mL
Ethyl 4-dimethylaminobenzoate solution....................................10.0mL
pH 7.0 ± 0.2 at 25°C
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin................................................................70.0mg
Preparation of Novobiocin Solution: Add novobiocin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Ethyl 4-dimethylaminobenzoate Solution:
Composition per 10.0mL:
Ethyl 4-dimethylaminobenzoate.................................................0.35g
Methanol......................................................................8.0mL
Preparation of Ethyl 4-dimethylaminobenzoate Solution:
Add ethyl 4-dimethylaminobenzoate to 8.0mL methanol. Mix thor-
oughly. Bring volume to 10.0mL with distilled/deionized water. Mix
thoroughly. Filter sterilize.
SLA-Octonoate Solution:
Composition per 50.0mL:
4-[2-(4-octanoyloxy-3,5-dimethoxyphenyl)-
vinyl]-quinolinium-1-(propan-3-yl
carboxylic acid) bromide
(SLPA-octanoate; bromide form)...............................................0.3223g
Preparation of SLA-Octonoate Solution: Add SLA-octonoate
to distilled/deionized water and bring volume to 50.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lution, SLA-octonoate solution, and ethyl 4-dimethylaminobenzoate
solution, to distilled/deionized water and bring volume to 920.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL no-
vobiocin solution, 50.0mL SLA-octonoate solution, and 10.0mL ethyl
4-dimethylaminobenzoate solution. Mix thoroughly. Pour into sterile
Petri dishes.
Use:  For the detection of Salmonella spp. in clinical specimens. For
the differentiation of Salmonella spp.