Cellulolytic Agar for Thermophiles

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulolytic Agar for Thermophiles

培养基配方
Composition per liter:
Agar..........................................................30.0g
K2HPO4 .......................................................1.65g
NH4SO4 ........................................................1.6g
Yeast extract..................................................1.0g
NaCl..........................................................0.96g
L -Cysteine·HCl·H2O..........................................0.5g
CaCl2 .......................................................0.096g
MgSO4 .......................................................0.096g
Cellulose suspension........................................200.0mL
Resazurin (0.1% solution) ....................................1.0mL
pH 7.2 ± 0.2 at 25°C
Cellulose Suspension:
Composition per 200.0mL:
Cellulose powder, Whatman CF11..................................8.0g
Preparation of Cellulose Suspension: Add cellulose powder to
200.0mL of distilled/deionized water and mix thoroughly.
Preparation of Medium: Prepare and dispense medium anaerobi-
cally in 100% N2 . Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2 with 5M
NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For cultivation of Clostridium stercorarium and other bacteria
that can utilize cellulose as a carbon source.