Castenholz Agar, Modified (DSMZ Medium 86a)

[所属分类:培养基配方] [发布时间:2020-4-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Castenholz Agar, Modified
(DSMZ Medium 86a)

培养基配方
Composition per liter:
Agar...................................................25.0g
NaNO3 .................................................0.69g
Na2HPO4 ...............................................0.14g
KNO3 .................................................0.103g
MgSO4 ·7H2O............................................0.1g
Nitrilotriacetic acid...................................0.1g
CaSO4 ·2H2O...........................................0.06g
NaCl...................................................8.0mg
MnSO4 ·H2O............................................2.2mg
ZnSO4 ·7H2O...........................................0.5mg
H3BO3 .................................................0.5mg
FeCl3 ................................................0.47mg
CoCl2 ·6H2O..........................................46.0μg
CuSO4 ·5H2O..........................................25.0μg
Na2MoO4 ·2H2O........................................25.0μg
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add nitrilotriacetic acid to 500.0mL of dis-
tilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add re-
maining components. Mix thoroughly. Readjust pH to 7.8. Bring volume
to 1.0L with distilled/deionized water. Mix thoroughly. Gently heat and
bring to boil. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
plates or aseptically distribute to sterile tubes or flasks.
Use: For the cultivation of Meiothermus taiwanensis.