Bordet-Gengou HiVeg Agar Base with 1.6% Agar

[所属分类:培养基配方] [发布时间:2019-12-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bordet-Gengou HiVeg Agar Base with 1.6% Agar

培养基配方
Composition per liter:
Potatoes, infusion from....................................125.0g
Agar........................................................16.0g
Plant peptone ..............................................10.0g
NaCl.........................................................5.5g
Rabbit blood..............................................200.0mL
Glycerol ..................................................10.0mL
pH 6.7± 0.2 at 25°C
Source: This medium without glycerol and blood is available as a pre-
mixed powder from HiMedia.
Preparation of Medium: Add 10.0g of glycerol to 790.0mL of dis-
tilled/deionized water. Add other components, except rabbit blood, to
the glycerol solution. Mix thoroughly. Heat with occasional agitation
of the medium. Boil for 1 min. Autoclave for 15 min at 15 psi pressure–
121°C. Cool medium to 50°C. Aseptically add 200.0mL of rabbit
blood (prewarmed to 35°C). 150.0–200.0mL of sterile, defibrinated
horse blood may be used in place of rabbit blood. Mix thoroughly and
pour plates or prepare slants.
Use: For the detection and isolation of Bordetella pertussis and Bor-
detella parapertussis from clinical specimens. Bordetella pertussis
appears as small (<1mm), smooth, pearl-like colonies surrounded by a
narrow zone of hemolysis. Bordetella parapertussis appears as brown,
nonshiny colonies with a green-black coloration on the reverse side.
Bordetella bronchiseptica appears as brown, nonshiny, moderately
sized colonies with a roughly pitted surface.