Bolton Broth

[所属分类:培养基配方] [发布时间:2019-12-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bolton Broth

培养基配方
Composition per 505mL:
Bolton selective enrichment broth base.......................500.0mL
Horse blood, lysed............................................25.0mL
Bolton selective supplement soution............................5.0mL
pH 7.4 ± 0.2 at 25°C
Bolton Selective Enrichment Broth Base:
Composition per liter:
Peptone .....................................................10.0g
Lactalbumin hydrolysate ......................................5.0g
Yeast extract.................................................5.0g
NaCl..........................................................5.0g
α-Ketoglutarate..............................................1.0g
Na-pyruvate ..................................................0.5g
Na-metabisulfite .............................................0.5g
Na2CO3 .......................................................0.6g
Hemin .......................................................0.01g
Preparation of Bolton Selective Enrichment Broth Base:
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C.

Bolton Broth Supplement Solution:
Composition per 5.0mL
Vancomycin ...............................................10.0mg
Cefoperazone..............................................10.0mg
Trimethoprim..............................................10.0mg
Cycloheximide.............................................10.0mg
Ethanol...................................................2.5mL
Preparation of Bolton Supplement Solution: Add antibiotics to
2.5mL ethanol. Mix thoroughly. Bring volume to 5.0mL with distilled/
deionized water. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Aseptically combine 500.0mL warm
Bolton selective enrichment broth base, 25.0mL lysed horse blood, and
5.0mL Bolton slective supplement soution.
Use: For the enrichment of Campylobacter spp. from foods.