Baird-Parker Egg Yolk Agar (ISO)

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baird-Parker Egg Yolk Agar (ISO）

培养基配方
Composition per 1050.0mL:
Agar........................................................20.0g
L -Glycine .................................................12.0g
Pancreatic digest of casein.................................10.0g
Sodium pyruvate.............................................10.0g
Meat extract.................................................5.0g
LiCl.........................................................5.0g
Yeast extract................................................1.0g
Egg yolk tellurite enrichment .............................50.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin wash with plenty of water imme-
diately.
Egg Yolk Tellurite Enrichment:
Composition per 100.0mL :
Chicken egg yolks.................................................10
K2TeO3 ........................................................0.15g
NaCl(0.9% solution)...........................................50.0mL
Preparation of Egg Yolk Tellurite Enrichment:  Soak eggs with
1:100 dilution of saturated mercuric chloride solution for 1 min. Crack
11 eggs and separate yolks from whites. Mix egg yolks. Measure
30.0mL of egg yolk emulsion and add to 70.0mL of 0.9% NaCl solu-
tion. Mix thoroughly. Add 0.15g K2 TeO3 . Filter sterilize. Warm to 45°–
50°C.
Caution: Potassium tellurite is toxic.
Preparation of Medium:  Add components, except egg yolk tellu-
rite enrichment, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 50 mL of egg yolk tellurite enrichment. Mix well. Pour into sterile
Petri dishes or sterile tubes.
Use : For the selective isolation and enumeration of coagulase-positive
staphylococci. A selective medium for the isolation and enumeration of
coagulase-positive staphylococci from food, with formulation con-
forming to that recommended in ISO 6888-1:1999.