Bacteroides Bile Esculin Agar (BBE Agar)

[所属分类:培养基配方] [发布时间:2019-12-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacteroides Bile Esculin Agar
(BBE Agar)

培养基配方
Composition per liter:
Oxgall .....................................................20.0g
Pancreatic digest of casein.................................15.0g
Agar........................................................15.0g
Papaic digest of soybean meal................................5.0g
NaCl.........................................................5.0g
Esculin......................................................1.0g
Ferric ammonium citrate......................................0.5g
Gentamicin solution..........................................2.5mL
Hemin solution...............................................2.5mL
Vitamin K 1 solution.........................................1.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Gentamicin Solution:
Composition per 10.0mL:
Gentamicin....................................................0.4mg
Preparation of Gentamicin Solution:  Add gentamicin to 10.0mL
of distilled/deionized water. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin ..........................................................0.5g
NaOH (1N solution)............................................10.0mL
Preparation of Hemin Solution:  Add components to 100.0mL of
distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................1.0g
Ethanol.......................................................99.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except hemin solution,
gentamicin solution, and vitamin K 1 solution, to distilled/deionized
water and bring volume to 994.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 2.5mL of sterile hemin solution, 2.5mL
of sterile gentamicin solution, and 1.0mL of sterile vitamin K 1 solution.
Use: For the selection and presumptive identification of the Bacteri-
odes fragilis group. For the differentiation of Bacteroides species
based on the hydrolysis of esculin and presence of catalase. After incu-
bation for 48 hr, bacteria of the Bacteroides fragilis group appear as
gray, circular, raised colonies larger than 1.0mm. Esculin hydrolysis is
indicated by the presence of a blackened zone around the colonies.