Bacillus acidoterrestris Broth

[所属分类:培养基配方] [发布时间:2019-11-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus acidoterrestris Broth
Composition per 1001.0mL:
Solution A..............................................................1.0L
Solution B (Trace elements solution SL-6)..............................1.0mL
pH 4.0 ± 0.2 at 25°C
Solution A:
Composition per liter:
Glucose ................................................................5.0g
KH2PO4 .................................................................3.0g
Yeast extract...........................................................2.0g
MgSO4 ·7H2O............................................................0.5g
CaCl2 ·2H2O...........................................................0.25g
(NH4)2SO4 ..............................................................0.2g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.0. Au-
toclave for 15 min at 15 psi pressure–121°C.
Solution B (Trace Elements Solution SL-6):
Composition per liter:
MnCl2 ·4H2O.............................................................0.5g
H3BO3 ...................................................................0.3g
CoCl2 ·6H2O ............................................................0.2g
ZnSO4 ·7H2O............................................................0.03g
NiCl2 ·6H2O............................................................0.02g
CuCl2 ·2H2O ...........................................................0.01g
Preparation of Solution B (Trace Elements Solution SL-6)
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 1.0L of sterile solu-
tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Bacillus acidoterrestris,
Alicyclobacillus acidoterrestris, and Alicyclobacillus cycloheptanicus.