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Oxytetracycline Glucose Yeast Extract Agar (OGYE Agar)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxytetracycline Glucose

Yeast Extract Agar

(OGYE Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................12.0g

Yeast extract........................................................5.0g

Biotin ...............................................................0.1mg

Oxytetracycline solution............................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Oxytetracycline Solution:

Composition per 10.0mL:

Oxytetracycline...................................................0.1g

Tris(hydroxymethyl) aminomethane

buffer (0.1M, pH 7.0) ............................. 10.0mL

Preparation of Oxytetracycline Solution:

Add oxytetracycline to 10.0mL of Tris buffer. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept oxytetracycline solution, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 10

min at 10 psi pressure–115°C. Cool to 45°–50°C.

Aseptically add sterile oxytetracycline solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation, enumeration, and cultivation

of yeasts and other fungi from foods.