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Oxidative-Fermentative Test Medium (OF Test Medium) (BAM M117)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidative-Fermentative Test Medium

(OF Test Medium)

(BAM M117)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl....................................................................5.0g

Agar ....................................................................2.5g

Peptone ...............................................................2.0g

K2HPO4...............................................................0.3g

Bromthymol Blue .............................................0.03g

Carbohydrate solution................................. 100.0mL

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes in

3.0mL volumes. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add

0.3mL of sterile carbohydrate solution to each tube.

Mix thoroughly.

Use: For the cultivation and differentiation of a vari

ety of microorganisms based on their ability to fer

ment a specific carbohydrate. Bacteria that ferment

the specific carbohydrate turn the medium yellow.