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Oxidative-Fermentative Glucose Medium, Semisolid with NaCl (OF Glucose Medium, Semisolid with NaCl) (BAM M116)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidative-Fermentative

Glucose Medium, Semisolid with NaCl

(OF Glucose Medium,

Semisolid with NaCl)

(BAM M116)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Glucose .............................................................10.0g

Agar ....................................................................2.0g

Pancreatic digest of casein..................................2.0g

K2HPO4...............................................................0.3g

Bromthymol Blue dye.......................................0.08g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For differentiating Gram-negative halophilic

Vibrio spp. based upon determining the oxidative and

fermentative metabolism of glucose. Bacteria that

ferment glucose turn the medium yellow. For

Campylobacter detection prepare half of the tubes

with glucose and half without.