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Ornithine Broth (BAM M44)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Ornithine Broth

(BAM M44)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

L-Ornithine..........................................................5.0g

Peptone or gelysate .............................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Bromcresol Purple ............................................0.02g

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH so that is will be 6.5 ± 0.2 after

sterilization. Distribute into 16 × 150mm screw

capped tubes in 5.0mL volumes. Autoclave medium

with loosely capped tubes for 10 min at 15 psi pressure–

121°C. Screw the caps on tightly for storage and after

inoculation.

Use: For the cultivation and differentiation of bacte

ria based on their ability to decarboxylate the amino

acid ornithine. Bacteria that decarboxylate ornithine

turn the medium turbid purple.