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Organic Acid Medium KP (Organic Acid Medium, Kauffmann and Petersen)

[所属分类:培养基配方] [发布时间:2021-12-7] [发布人:网站管理员2] [阅读次数:] [返回]

Organic Acid Medium KP

(Organic Acid Medium,

Kauffmann and Petersen)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin...............................................................10.0g

Bromthymol Blue ...........................................0.024g

Organic acid solution .................................. 100.0mL

pH 7.4 ± 0.2 at 25°C

Organic Acid Solution:

Composition per 100.0mL:

Organic acid......................................................10.0g

Preparation of Medium: Add organic acid to

distilled/deionized water and bring volume to

100.0mL. Sodium potassium D-tartrate, sodium cit

rate, or mucic acid may be used. Mix thoroughly

Preparation of Medium: Add components, ex

cept organic acid solution, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile organic acid solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and differentiation of mem

bers of the Enterobacteriaceae based on their ability

to utilize different organic acids as carbon source.

Bacteria that utilize tartrate, citrate, or mucate turn

the medium yellow.