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Nutrient Broth Salts Medium

[所属分类:培养基配方] [发布时间:2021-12-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

NaCl....................................................................3.0g

MgSO4·7H2O......................................................0.2g

CaCl2·2H2O.......................................................0.15g

MnSO4·H2O......................................................0.05g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacillus

subtilis.