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Nutrient Agar with 0.5% NaCl, pH 9.5-10.0 (LMG Medium 253)

[所属分类:培养基配方] [发布时间:2021-12-7] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar

with 0.5% NaCl, pH 9.5-10.0

(LMG Medium 253)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

NaCl..................................................................10.0g

Peptone .............................................................. 5.0g

Yeast extract........................................................2.0g

Lab-Lemco beef extract......................................1.0g

pH 9.5-10.0

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Adjust

pH to 9.5-10.0 with sterile Na2CO3 solution. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus

spp.