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VRE Broth

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VRE Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

Calf brain infusion solids............................................................ 12.5g

Proteose peptone ......................................................................... 10.0g

Beef heart infusion solids ............................................................. 5.0g

NaCl .............................................................................................. 5.0g

Na2HPO4 ....................................................................................... 2.5g

Glucose ......................................................................................... 2.0g

Selective supplement solution ...................................................4.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Selective Supplement Solution:

Composition per 4.0mL:

Meropenum................................................................................ 2.0mg

Preparation of Selective Supplement Solution: Add meropenum

to distilled/deionized water and bring volume to 4.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except selective sup

plement solution, to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asep

tially add 4.0mL selective supplement solution. Mix thoroughly .

Aseptically distribute into sterile tubes.

Use: For the isolation of high-level aminoglycoside-resistant entero

cocci (HLARE) from clinical samples. Nonresistant enterococci will

not grow on this medium. The selective supplement suppresses growth

of Gram-negative bacteria and E. gallinarum.